PJB-2018-726
Protein and isozymes based genetic diversity analysis within Solanum genus
Mesfer M. Alqahtani
Abstract
Biodiversity analysis on thirteen genotypes representing six different species of Solanum (S. torvum, S. dulcamara, S. villosum, S. nigrum, S. incanum and S. sisymibriifolium) was applied using three biomarkers; morphological characters, seed storage protein and isozymes. The plant samples were collected from different five areas in Aseer region, south west of Saudi Arabia and morphologically described. Total protein content in dry seeds of different Solanum genotypes were extracted and analyzed using SDS-PAGE. Alchol dehydrogenase (ADH) and malate dehydrogenase (MDH) isozymes were extracted and detected. Twelve out of eighteen qualitative and quantitative morphological characters were polymorphic among the thirteen Solanum genotypes, reflecting the difficulty to choose the most informative morphological characters. Twenty one polymorphic protein bands out of thirty five were scored. ADH isozyme showed low level of variation with four polymorphic enzymatic bands, while, MDH isozyme showed relatively higher level of polymorphism in which 10 polymorphic out of total 12 enzymatic bands were scored across different Solanum genotypes. Data from the three biomarker did not support the separation of genotypes of S. nigrum and S. villosum into different subspecies. Data would also suggest that little morphological variations within genotypes could be confined to different habitats and geographical areas. Our results revealed that, protein electrophoresis and isozymes analysis can be used to demonstrate the genetic biodiversity between Solanum species.
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