PJB-2018-736
Effect of cold stress on gene expression and functional pathways in maize root system
Yanhua Ma
Abstract
Objective: This study aimed to explore the key genes and functions involved in the cold response of primary maize under low temperature. Methods: The gene expression profiling GSE72508 was downloaded from Gene Expression Omnibus database. The genes that were differentially expressed in maize primary root under control and cold conditions were screened. These genes were performed functional enrichment analyses and protein-protein interaction (PPI) network establishment. Furthermore, the DEGs involved in PPI network were conducted principal component analysis (PCA) to further screen key genes induced by cold stress. Results: Total 127 differentially expressed genes were screened between cold treatment and control groups. They were significantly enriched in functions of polysaccharide metabolic and biosynthetic processes, carbohydrate biosynthetic process, and chloroplast, as well as pathway of zma00500:Starch and sucrose metabolism. PPI network containing 38 gene nodes and 75 edges was constructed. PCA analysis identified five key genes, like LOC100284362, RPL16 and RPL14. Conclusion: Cold stress may influence the normal growth and development of maize through changing the metabolism and biosynthesis of sucrose and carbohydrate, as well as affecting the functions of chloroplast. RPL16 and RPL14 may be key genes involved in cold response of maize under low temperature.
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