PJB-2018-753
Biochemical analysis and comparative studies on Diospyros malabarica (Gaab Tree)
Zohra Zreen
Abstract
Analysis on potential use of Diospyros malabarica was missing to date in the biochemical figure of Pakistani plants. Keeping in mind the ascribed value of D. malabarica, the current study was undertaken to observe antioxidant potential of D. malabarica for health promoting and pharmaceutically important phytochemicals to fight against many disorders. Biochemical analysis of different plant organs of D. malabarica viz enzymatic, non enzymatic antioxidants hydrolytic enzymes and few other biochemical parameters such as total antioxidant activity, total oxidant status, soluble sugars and protein along with pigments like chlorophyll ( total, a and b), carotenoids and lycopene were studied. In our research findings, highest level of α-amylase, ascorbate peroxidase, tannins, esterase, reducing and non-reducing sugar and lowest total oxidant status were observed in bark. Unripe fruit showed significant activities of superoxide dismutase, catalase and highest total phenolic contents. Significant amount of ascorbic acid, peroxidase, total soluble sugars and proteins were studied in ripe fruit while protease and pigments like chlorophyll (total, aand b), lycopene and carotenoids were investigated in leaves. D. malabarica stem did not show significant results. Our present findings concluded that leaves, ripe and unripe fruit of D. malabarica could be capitalized in pharmaceutical applications due to significant presence of different antioxidants but abundance of antioxidants in bark extract make it more valuable and potent therauptic agent. It is interesting to note that D. malabarica stem did not show any remarkable result. According to the above facts and findings, further biochemical and pharmacological investigations are required to find out natural products. Keywords: Diospyros malabarica, enzymatic antioxidants, non-enzymatic antioxidants, Biochemical analysis, Indigenous medicinal plant, herbal drugs.
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