PJB-2018-754
EFFECTS OF COMBINED NACL AND DROUGHT IN TWO PEA CULTIVARS
Houneida Attia
Abstract
The purpose of the work was to assess the combined effect of drought and salinity in two pea (Pisum sativum L.) cultivars cv Lincoln and cv Douce de Provence. Seedlings were cultured on liquid medium without or with NaCl, 75 mM in controlled conditions. Fourteen days old, salt treated plants were subjected to drought (PEG 6000). After 7 days of treatment, growth parameters, mineral content (Na+, Cl-, K+, Ca2+ and Mg2+) and chlorophyll content were measured. Peroxidases and catalase activities was assayed on gels. The combined action of drought and salt restricted leaf biomass more pronounced in cv D. Provence than in cv Lincoln. This growth decrease was accompanied by a restriction in leaf hydration and K+ uptake, concomitant with low Na+ accumulation in cv D. Provence leaves. Considering the response of antioxidant enzymes to combined stress, leaves guaiacol peroxidase activity showed an increase as compared to control in much more pronounced in cv Lincoln than in cv D. Provence. Both pea cultivars exhibited a stimulation of catalase activity with higher in cv D. Provence (osmotic stress sensitive) than in cv Lincoln (less osmotic stress sensitive).
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