PJB-2018-760
OPTIMIZING CALLUS INDUCTION, REGENERATION AND GROWTH PARAMETERS AND GENETIC TRANSFORMATION OF JP-5 RICE FOR ABIOTIC STRESSES
Qismat Shakeela
Abstract
In Pakistan rice is the most edible food after wheat and as an export item it has major contribution in the country’s foreign reserve. In present study, JP-5 was used with different concentrations of 2.4-D and casein hydrolysate in two different growth media i.e. MS and N6. Regeneration of callus was checked by using different concentrations of BAP (Benzyleaminopurine). Maximum callus induction was found to be 89% and 87% with MS and N6 media respectively. Statistical analysis by Fishers exact test revealed that casein concentration of 300 mg/ml significantly (P>0.011) enhanced the callus induction as compared to other concentrations used in the study. In addition, chi square analysis showed overall association (P>0.056), also seems to be moderately significant. Similarly, maximum regeneration was observed to be 35% with BAP concentration of 300μg/ml. In order to get efficient transformation various factors with different concentrations necessary for transformation were probed. After 2-3 weeks of dryness the 26% of calli regenerated successfully. Transient gene expression was identified both at callus level and then after the plant growth via GUS expression test. Highest transformation efficiency was investigated to be 68.7%.
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