PJB-2019-36
Construction of expression vectors of the melon resistance gene Fom-2 and genetic transformation
Xiaomei Li, Liu Liu, Xiangyu Liu, Yan Hou, Bingyin Xu, Feishi Luan and Xuezheng Wang
Abstract
In this study, we constructed positive and negative expression vectors using the Melon Wilt Resistance Related Gene Fom-2 and transformed the vectors into melon via the pollen tube pathway. In the field, 52 Kan-resistant M4-45 plants and 84 Kan-resistant MR-1 plants were transformed with the Antisense Vector, and the conversion rates were 3.28% and 4.26%, respectively. The PCR results showed that antisense vectors were integrated into the genome of Cucumis melo. The results of a special inoculation test of Fusarium oxysporum melonis showed that the incidence of Fusarium wilt in transgenic plants was 84% and that in non-transgenic plants was 96%. The contents of chitinase, polyphenol oxidase and peroxidase increased with the bacterial treatment time in transgenic plants and non-transgenic plants, and the activities of these three enzymes in transgenic plants were higher than those in non-transgenic plants.
To Cite this article:
Li, X., L. Liu, X. Liu, Y. Hou, B. Xu, F. Luan and X. Wang. 2019. Construction of expression vectors of the melon resistance gene Fom-2 and genetic transformation. Pak. J. Bot.., 51(1): DOI: http://dx.doi.org/10.30848/PJB2019-1(36)
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