PJB-2018-791
GLUTAMIC ACID-INDUCED PHYSIOBIOCHEMICAL CHANGES IN BARLEY (Hordeum vulgare L.) UNDER SALT STRESS
SHAGUFTA PERVEEN
Abstract
Glutamic acid has been shown to alter chlorophyll contents and regulate growth in some crop species under normal conditions. The experiment was conducted to investigate the effect of foliar application of glutamic acid on physiobiochemical attributes of two barley (Hordeum vulgare L.) varieties (var. B-9008 and var. B-9006) under salt stress. Seeds of barley were collected from Ayub Agricultural Research Institute, Faisalabad and sown in sand filled plastic pots under natural climatic conditions. Two-week-old barley plants were subjected to two levels of salt (0 and 120 mM NaCl in full strength Hoagland's nutrient solution) stress. Three levels of glutamic acid i.e., 0, 10 and 20 mM were applied as foliar spray on 9-week-old barley plants under both salt stress and non-stress conditions. After two weeks of foliar application, barley (11-week-old) plants were harvested for determination of different growth, physiological and biochemical parameters. Salt stress of 120 mM NaCl significantly decreased shoot and root lengths, shoot dry weight, root fresh and dry weights and carotenoid contents, while increased chl. b (under salt stress), chl. a/b ratio (under non stress) in var. B9006, hydrogen peroxide (H2O2), total free amino acids, membrane permeability (%), free proline, glycinebetaine (GB), total soluble sugars, ascorbic acid, total phenolic, and anthocyanin contents in both barley varieties and flavonoid contents in the var. B9008. Foliar application of glutamic acid significantly increased root fresh and dry weights, shoot and root length, GB contents of both barley varieties and the total soluble sugars in var. B9006 under salt stress. The barley var. B-9008 showed good performance in terms of shoot and root lengths, total phenolics and total soluble protein contents, while var. B-9006 excelled in flavonoid and total soluble sugar contents irrespective of stress.
To Cite this article:
Download
