PJB-2018-814
Transcriptome sequencing of Pinus massoniana rooted and non-rooted cuttings
Fengjiao Zhou
Abstract
Pinus massoniana Lamb. is a unique and fat-producing tree species growing in southern China. However, cuttings of this species are difficult to root. In this study, transcriptome sequencing of rooted and non-rooted cuttings of this species was performed by Illumina sequencing technology. We obtained 138,466,788 raw reads and 132,448,884 clean reads including 124,396 unigenes after de novo assembly; these unigenes were annotated into seven databases. In the rooted and non-rooted cuttings, 781 differentially expressed genes (DEGs) were found, of which 368 were up-regulated and 413 were down-regulated. The Kyoto Encyclopedia of Genes and Genomes (KEGG) annotation indicated that these DEGs were enriched in 73 pathways, 18 of which were significantly enriched, including ‘photosynthesis’, ‘phenylpropanoid biosynthesis’, ‘starch and sucrose metabolism’, and ‘carbon fixation in photosynthetic organisms’. ‘Plant hormone signal transduction’ was involved in the regulation of endogenous hormones, including some genes related to IAA that were significantly associated with rooting such as AUX/IAA, ARF, GH3, SAUR, ERF1/2, MYC2, GID1, PP2C, and PR-1. These genes were also found in the rooting processes of other plants. Compared with the non-rooted Pinus massoniana seedlings, AUX/IAA was significantly down-regulated in the rooting shoots. We selected 10 genes from the DEGs and performed real-time quantitative polymerase chain reaction (PCR) verification. The results were consistent with the gene expression based on the RNA-seq technology. Our study may be useful for improving the rooting rate of Pinus massoniana cuttings.
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