PJB-2018-829
Molecular cloning of BeMYB140 from Bambusa eminencies, canvassing transactivation and expression profiling for its dynamic chunk against abiotic stresses
Muhammad Imran, Naseem Samo, Shanglian Hu, Xuegang Luo and Ying Cao
Abstract
The MYB is a major family of transcriptional factor’s (TFs), which significantly regulate several physiological processes in plants, i.e. growth, metabolism and development on one side and defenses against abiotic and biotic stresses on other. In this research a novel gene “BeMYB140” (GenBank accession number MG763923) encoded for TF was cloned and characterized from Bambusa emeiensis. The isolated gene BeMYB140 has an open reading frame (ORF) of 723bp which encoded a protein of 240 amino acids with a predicted relative molecular mass of 27.34346 kDa. Multiple proteins sequence alignment and phylogenetic analysis revealed that this protein contains a typical MYB domain and showed high homology with HvMYB1, ZmMYB38 and AtMYB4. Meanwhile, transactivation analysis and sub-cellular localization assay unveiled that the BeMYB140 protein is a transcriptional activator and localized in the nucleus. Moreover, BeMYB140 exhibited a dynamic expression under different abiotic stressors, such as, in response to ABA and Na2SO4 treatments down regulation was followed by a significant up-regulation; whereas the opposite results were observed during PEG treatment. The highly significant difference and maximum fold changes were observed in PEG, NaCl and H2O2 treatments. In ABA and NaCl treatments the peak expression level were observed during 3hrs, meanwhile, under PEG and H2O2 treatments BeMYB140 was strongly induced during later stages i.e. 24hrs. It is concluded from the results, that BeMYB140 involved in broad range of abiotic stresses and mediates stress tolerance in Bambusa emeiensis.
To Cite this article:
Imran, M., N. Samo, S. Hu, X. Luo and Y. Cao. 2021. Molecular cloning of BeMYB140 from Bambusa eminencies, canvassing transactivation and expression profiling for its dynamic chunk against abiotic stresses. Pak. J. Bot., 53(1): DOI: http://dx.doi.org/10.30848/PJB2021-1(36)
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