PJB-2018-843
An optimized protocol for in vitro propagation of Pyrus communis and Pyrus syriaca using apical-bud microcuttings
Mariem Lotfi
Abstract
Using apical explants, different media and plant growth regulators were tested to establish a micropropagation procedure for best local Tunisian Pyrus communis cultivars ‘Arbi’, ʻMaltiʼ, ʻMahdia 6ʼ and ʻMoknine 10ʼ and for Pyrus syriaca. Disinfection with 4% HgCl2 treatment for 20 minutes showed the highest percentage of plant survival. Successful initiation of the cultures was achieved on MS basic medium supplemented with 0.25 mg l-1 BA. During the proliferation stage, optimal shoot multiplication was obtained on MS medium with half the concentration of NH4NO3 and KNO3 supplemented with 0.1 mg l-1 IBA and 2 mg l-1 BA, but for maximum shoot length the BA concentration needed to be lowered to 1 mg l-1. A rooting rate of 100% and the highest root length and number were attained on Cheng medium supplemented with 1.0 mg l-1 IBA.
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