PJB-2018-884
MICROBIOLOGICAL STUDIES ON RHIZOBIUM LEGUMINOSARUM ISOLATED FROM PEA (PISUM SATIVUM) CROP OF QUETTA ZONE
FAROOQ SHAHZAD
Abstract
abstract Root nodules were collected from young and healthy seedling of Pisum sativum at different locations of Quetta zone, Baluchistan. Results revealed that 66.5% root samples were positive for Rhizobium leguminosarum while 33.5% were found negative. Out of positive samples 8% from Quetta, 18% from Pashin, 16% from Killhaabdulla, 13.5% from Hanna urak and 11% from Kuchlack. The PCR result shows clear bend of 1300 base pair 16sRNA gene under UV light. Rhizobium leguminosarum was further applied as a biofertilizer and trial results showed that plant height was increased 2.95 cm, fresh biomass was increased 1.87 gm, dry biomass was increased 2.49 gm and numbers of pods observed was 6 after biofertilizer treatment as compared to control group.Different medicinal plants trait showed that Rhizobium leguminosarum was sensitive to Cocculus pendulus (29mm), Malva neglecta (15mm), Rhazya stricta (11mm), Corchorus depressus (16mm), Salvia bucharica (12mm), Berberis baluchistanica (17mm) and Artemisa absinthium (13mm). The effect of various factors such as, temperature, pH, in vitro growth of Rhizobium leguminosarum indicated that the growth was highly influenced by low and high temperatures as well as pH. However, a remarkable growth of Rhizobium leguminosarum noted at 28oC to 30oC whereas, less growth was recorded at 24oC, 34oC, and 42oC. Similarly Rhizobium leguminosarum showed growth at pH 5 to 10 but, the superlative pH values for the growth of Rhizobium leguminosarum were from 6 to 8. Different insecticides were used in agriculture sector to increase the crop production. However only Chlorpyrifos and Cypermethrine were selected to evaluate its antimicrobial toxic effects on locally isolated Rhizobium leguminosarum. Cypermethrin exhibited least zone of inhibition 10 and 11 mm while Chlorpyrifos exhibited least zone of inhibition 14 and 17 mm with the highest dilution by using disc and well method.
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