PJB-2018-894
ISOLATION AND SCREENING OF FREE LIVING RHIZOBACTERIA FROM ALHAGI MAURORUM FOR THEIR POTENTIAL ROLE IN PHYTOHORMONE PRODUCTION AND GROWTH ENHANCING ABILITY
Hina Gul
Abstract
The (PGPR) occupying the rhizosphere, are the great effective groups of bacteria, which advantage the crop plants in numerous methods. Salt-tolerant plants are known as halophytes located absolutely in environments with great extents of soil salinity. With the objectives of isolating PGPR from rhizosphere of halophyte plants, their biochemical characterization, ability to tolerate salt stress, PGP abilities such as IAA and Cytokinin production, Nitrogen fixation and Phosphate solublization and effect of these strains on germination and seedling growth of maize and mung bean. A total of 2rhizobacterial strains were isolated from halophyte Alhagimaurorum. All isolates were gram positive bacteria. Both the isolates exhibited indole acetic acid (IAA) and cytokinins production and none of the isolates shown nitrogen fixing ability in semi-solid malate medium, also none of the isolate shown phosphate solubilization ability in Pikoviskaya medium. Auxins concentration was maximum for isolate Am2 that was 211.8406µg/ml while maximum cytokinins was produced by isolate Am2 (256.958µg/ml)respectively. Maximum salt tolerance was indicated by isolateAm1 that grows upto 4% NaCl concentration. The germination assay experiment after seven days of growth and development showed that most of the isolates could increase root and shoot length of seedlings as well as fresh and dry biomasses as compared to control. Similarly, seedling growth of Vigna radiate (mung bean) was tested by conducting an experiment in sand culture in pots. After 15 days of growth chlorophyll content, shoot length, root length, fresh and dry biomasses showed that as compared to control most of the bacterial isolates meaningfully improved plant growth. This study suggests that isolates with higher growth activities can also be used further as bio-fertilizers for different crops under field trials.
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