PJB-2018-899
L-asparaginase, acrylamide quenching enzyme production from leaves of Tamarindus indica and seeds of Vigna radiata–Fabaceae
Ambreen Aisha, Muhammad Anjum Zia, Muhammad Asgher and Faqir Muhammad
Abstract
Current study aims at high yield production of L-Asparaginase from family fabaceae. Leaves of Tamarindus indica, seeds of Vicia faba, Phaseolus vulgaris, Pisum sativum, Cicer arientinum, Vigna radiata are screened for l-Asparaginase yield and activity. Prepared crude extracts are purified through ammonium sulfate precipitation, dialysis, ion exchange chromatography and gel filtration. When incubated with l-Asparagine, crude extracts of Pisum sativum leaves and seeds of Cicer arientinum, Phaseolus vulgaris and Vicia faba extracts showed 24.93 U/mL, 10.38 U/mL, 11.81U/mL enzymatic activity, respectively. Crude extract of Tamarindus indica leaves exhibited highest enzyme activity of 64.38 U/mL, with a yield of 67% while Vigna radiata seeds crude extract revealed 53% yield with enzymatic activity of 56.34 U/mL.l-Asparaginase enzyme purified from Tamarindus indica has activity of 54.260±0.847 U/mL with specific activity of 95.67 U/mg. l-Asparaginase purified from Vigna radiata seeds exhibit 45.140±0.302 U/mL activity and specific activity 135.25 U/mg. The purified enzyme exhibits maximal activity at 37℃, stability at optimal pH 8.6 in the presence of alkaline 0.2 M potassium phosphate buffer. Optimal substrate concentration for l-Asparaginase activity is 3.5 ug/mL from Tamarindus indica. Results shows that Vigna radiata and Tamarindus indica can be a better choice as high source of l-Asparaginase that can be employed to reduce acrylamide cancer provoking element in food industry
To Cite this article:
Aisha, A., M.A. Zia, M. Asgher and F. Muhammad. 2019. L-asparaginase, acrylamide quenching enzyme production from leaves of Tamarindus indica and seeds of Vigna radiata–Fabaceae. Pak. J. Bot., 52(1): DOI: http://dx.doi.org/10.30848/PJB2020-1(42)
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