PJB-2018-922
IN VITRO ANTIFUNGAL EFFECTS OF FUMARIA VAILLANTII AND F. ACEPALA PHENOLS ON ASPERGILLUS FLAVUS AND A. NIGER
MONIR MOHSENI
Abstract
Fumaria vaillantii and F. acepala belonging to the family Fumariaceae have been traditionally used. Aspergillus affects the human diet by poisoning the fruits and vegetables. F.vaillantii and F. acepala were collected from Saveh of Iran in 2017. Phenolic compounds were extracted and measured using Folin-Ciocalteu assay. Phenols were analyzed by GC-MS. Phenol added in culture medium of Aspergillus for antifungal activity tests. The total phenol of F. vaillantii and F. acepala were 9.3 and 9.76 mg.g-1dw without significant difference. 8 phenolic compounds in F.vaillantii and 3 phenolic compounds in F. asepalla were identified by GC-MS analysis. Algrain, quercitrin, Kaemferol and Rutin of F.vaillantii. Phenol of F. vaillantii and F. asepalla give good antifungal effects against A. flavus and A. niger. Antifungal effects of F. vaillantii and F. acepala phenols were similar and without significant difference. F. vaillantii and F. acepala phenols with the effect on the fungal cell membranes and induction of plasmolysis caused the loss of water, induced thinning and breaking of the walls in some hypha, preventing fungal growth. Phenolic compounds of both species of fumaria had antifungal effect with effect on cell membrane and plasmolysis induction, thinning and braking of the walls and disintegration of hypha building.
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