PJB-2018-930
POTENTIAL OF LECANICILLIUM LECANII (VERTICILLIUM LECANII) AS MICROBIAL CONTROL AGENT OF GREEN PEACH APHID (MYZUS PERSICAE SULZER)
Abdul Hanan
Abstract
The utilization of entomopathogenic fungi as potential microbial control against different insect pests is important due to their eco-friendly nature. In-vitro studies, the virulence potential of 3 different isolates of Lecanicillium lecanii formerly recognized as Verticillium lecanii (V-2, V-3 and V-5) with different bioassay materials (conidia, filtrate and binary combination of conidia + filtrate) were evaluated against green peach aphid (Myzus persicae). Conidial bioassay of fungal isolates were evaluated with three different concentrations (1×106, 1×107 and 1×108), filtrate bioassay was estimated by applying 7th day fungal filtrate and binary combination (1ml conidia +1ml filtrate) of (V2×V2), (V3×V3) and (V5×V5) were tested against green peach aphid (Myzus persicae). In conidial bioassay, V-3 showed maximum aphid mortality (95%) and V-5 showed the lowest mortality (70%) at the highest concentration (1×108 per ml conidia) at 8th day post treatment whereas in filtrate bioassay, 98% mortality was achieved in V-3, and in V-5, recorded mortality up-to 74%. Similarly in binary combination of fungal conidia 1×108 conidia per ml and its filtrate, combination (V-3×V-3) revealed the highest aphid mortality 91% while combinations, (V-2×V-2) and (V-5×V-5) resulted in lowest aphid mortality 79% and 65% respectively. The results revealed that in all types of bioassays, V-3 isolate was the most virulent and its filtrate application was found to be the most effective against peach aphid.
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