PJB-2018-935
INDUCTION OF CALLUS AND PLANT REGENERATION FROM LEAVES OF BRYOPHYLLUM AND EFFECT OF SALT STRESS ON CALLUS.
OMAR H. OBAID
Abstract
In this investigation, Callus obtained from Bryophyllum leaves on MS media with different concentrations of 2, 4-D (1, 2, 3) mgl-1 and containing sucrose and vitamins. The callus re-differentiation on the same medium with modifying growth regulators Kn (1.0, 0.5) mgl-1 and IAA (0.5, 0.1) mgl-1, the effect of salt stress on the callus cells level and at different salinity levels (10%, 20%, 30%, 40%) was studied for 28 days. Results showed that, the best concentration for callus production was in the concentration of 2 mgl-1 2,4-D and regeneration of the callus to, plantlets with Kn 1.0 mgl-1 and IAA 0.1 mgl-1, with MS media. The results showed that Bryophyllum callus was able to tolerance to salt stress up to 30% concentration of NaCl. Although wet and dry weight were affected by salt stress from the first concentration 10%.
To Cite this article:
Download
