PJB-2018-942
FIRST PROTEOMIC ASSAY OF PAKISTANI LANDRACES OF COMMON BEANS (PHASEOLUS VULGARIS) CULTIVATING IN THE HILLY AREAS OF MALAKAND DIVISION, KHYBER PAKHTUNKHWA, PAKISTAN
khan Sher
Abstract
Common-bean is an important food source in Pakistan, being grown in the hilly areas of country. In general practice, the plants of common beans are grown from seed collected locally from the previous year’s crops which are genetically adapted to the local environmental conditions. These local collections or landraces are in danger of being replaced by genetically uniform commercial varieties before their genetic diversity and breeding potential has been assessed. In this activity, the diversity of 117 common bean landraces was assessed through seed storage protein profile using SDS-PAGE. The electrophoregram exhibited two zones. R-I, contained six bands ranging in between 180 to 34kDa and RII comprised of 4 bands ranging in size from 33 to 10kDa. The average Genetic Index for R-I was 17% while for R-II it was 27%. Among all bands, B-4 and B-5 had the greatest Genetic Diversity (90.59% and 88.88%, respectively). Dendrogram based on R-I and R-II sorted all the landraces into 13 and 10 clusters respectively showed a high level of GD within landraces. High degree of Variation in protein bands profile and multiple clustering validate that in future Pakistani bean landraces will provide an abundant chance for obtaining genetically strong and stable genotype with high valued production to breeders. Furthermore, 2-D electrophoresis and DNA based markers studies are recommended to study the genetic diversity of Pakistani bean landraces
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