PJB-2018-947
DIFFERENTIAL EXPRESSION OF PINORESINOL-LARICIRESINOL REDUCTASE GENE IN RELATION TO PODOPHYLLOTOXIN ACCUMULATION IN DIFFERENT ORGANS OF ENDANGERED ANTICANCER SPECIES PODOPHYLLUM PELTATUM
Wei Liu
Abstract
Pinoresinol-lariciresinol reductase (PLR) is a key enzyme in biosynthesis pathway of anticancer podophyllotoxin. PLR catalyzes the conversion of pinoresinol into secoisolariciresinol, a central precursor for podophyllotoxin biosynthesis. Various organs of Podophyllum peltatum that is an important anticancer species contain podophyllotoxin, however podophyllotoxin content significantly varys with different organs. Therefore, investigation of PLR gene expression in different organs facilitates to understand the molecular mechanisms of podophyllotoxin content differences in different organs and its biosynthesis, and suggest an important gene for metabolic engineering the podophyllotoxin in P. peltatum. The podophyllotoxin were detected in all the tested organs (root, rhizome, petiole, fruit, leaf and flower), and siginificant differences were observed among them (P<0.05). The highest podophyllotoxin content (2.8018 ± 0.23%) was found in rhizome that is 30 times higher than that in fruit (the lowest). Furthermore, tissue expression profile showed that P. peltatum PLR (PpPLR) was expressed only in 5 organs, but without in flower. The highest and lowest expression level was found in rhizome and in leaf, respectively. The expression profile of PpPLR was not consistent with the podophyllotoxin content in tested organs except for the rhizome. This means that the podophyllotoxin biosynthetic organ is not necessarily its storage organ, and the storage organ is probably the rhizome.
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