PJB-2018-990
Proton gradient regulator 5 (gaPGR5) of Gossypium arboreum enhances salt-stress tolerance in Gossypium hirsutum
Muhammad Naveed Shahid
Abstract
Cotton is most important fibrous and cash crop cultivated in more than fifty countries of the world. Cotton crop yield is seriously affected by soil salinity. This deleterious effect can be reduced by genetic modification in stress susceptible cotton plant. Salt stress tolerant gene gaPGR5 (proton gradient regulator) was isolated from Gossypium arboreum and transformed into the stress- susceptible cotton cultivar (G. hirsutum). gaPGR5 gene was cloned into pCAMBIA-1301 vector and the cotton young embryos were used to transform by Agrobacterium-mediated method. Plant Gus gene was used as reporter gene that showed blue coloration during histochemical assay. Molecular analysis of transgenic plants was done up to T2 generation. Selection of salt-tolerant transgenic plants was done by salt-stress (NaCl) treatment with different concentrations in hydroponic culture. Transgene expression in salt-tolerant transgenic plants was evaluated through quantitative real-time PCR. Maximum transgene expression was recorded in those plants which were tolerance to higher salt concentration (175mM NaCl) and vice versa. The plants which gave higher transgene expression against salt stress are valuable for cultivation in salt-affected land areas.
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