PJB-2018-1005
Study of relative frequency and spectrum of chlorophyll mutants in M2 generation for facilitating mutation breeding
Irem Waqar
Abstract
Four genotypes of chickpea, two desi type (03009 & 07041) and two kabuli type (0709 & CC98/99) were used to study the efficiency and effectiveness of different mutagens (EMS and gamma rays) and their doses (200, 300, 400 & 500 Gy of gamma radiations & 0.1%, 0.2%, 0.3% and 0.4% EMS). Relative frequency and spectrum of chlorophyll mutants in M2population were studied. The overall relative frequency of different types of mutants was as xanthan-17% > viridis-4.22% > chlorina-0.95% >albino-0.13% respectively. EMS was observed more effective mutagen than gamma radiation. Further 0.3% dose of EMS gave best results with maximum frequency of chlorophyll mutants i.e. 7.72% while albino was least frequent with 0.13% in all genotypes. A desi genotype 07041 was most responsive to mutagenesis. It is clear that each mutagen and their doses produced different relative frequency and spectrum of chlorophyll mutants, so all these mutagen and doses should be included for induction of viable mutants, which facilitate our mutation breeding programs
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