PJB-2018-1040
COMPARATIVE STUDY OF BIO-CULTURING OF FRESH WATER ALGAE, SPIROGYRA AND HYDRODICTYON
MEHwISH JAFFER
Abstract
he research was commenced with the optimization of growth cultural conditions for algal biomass production of two local filamentous fresh water species, Hydrodictyon reticulatum, and Spirogyra communis. These algal samples were gathered from fresh water bodies of Lahore. These species were then refined in glass container having 1000ml of culturing media, A-Z media, Bristol’s soil media and Bristol’s media were assessed for production of algal biomass. Amongst all these tested media, Hydrodictyon gave maximum biomass (18.60g/L) in Bristol media whereas, Spirogyra gave the biomass of 10.5g/L in Bristol soil media.. Afterward the consequence of different Carbon sources (Lactose, Glucose, Cellulose and Starch) supplement in Bristol media were evaluated for biomass production. Among all the sources tested Cellulose at 1g/L was found to be significant for optimal mass production of (15.81g/1L) and (18.6 g/1L) of Spirogyra and 1lHydrodictyon respectively. Both algal species gave insignificant results in all other carbon sources. Then the impact of various nitrogen sources (Ammonium nitrate, Ammonium Sulphate, Ammonium Chloride, Potassium Nitrate and Urea) were tested for biomass production. Among all the sources tested urea at 0.1 g/L was found to be best for optimal growth (20.7g/1000ml) of Hydrodictyon as compared to (16.86g/1000ml) of Spirogyra. However, cellulose as carbon source and urea as nitrogen source was optimized for significant growth of both the algal species. The comparison between the growth rates of both specimens was evaluated and it was concluded that both the species gave maximum growth upto 15 days of incubation and then the growth start decreasing gradually. The statically parametric studies indicated that the volumetric growth of Hydrodictyon is significant in the selected media as compared to Spirogyra.
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