PJB-2018-1042
ESTIMATION OF GENETIC DIVERSITY IN DIALLEL CROSSES OF MAIZE GENOTYPES USING SIMPLE SEQUENCE REPEAT (SSR) PRIMER SETS
zakiullah
Abstract
Genetic diversity is an essential element for maize breeding. High level of genetic diversity in maize population is an indication of valuable maize resource for successful hybrid breeding programmes. SSR markers were shown to be a powerful tool for detection of genetic diversity in maize population. The research project was conducted to derivate genetic diversity among 36 maize genotypes using 25 simple sequence repeat (SSR) primer sets. The range of genetic diversity were <100-850> bp. 100 out of 129 loci/bands were polymorphic with 25 SSR markers nd remaining 29 loci/bands exhibits monomorphic results. Monomorphic and polymorphic results were given in figures. To find out the genetic diversity among the 36 maize genotypes, bivariate data matrix was generated and genetic distances were calculated. High degree of genetic polymorphism was observed among the 36 maize genotypes with overall genetic distance ranged from 0-100% and average genetic distance ranged from 0 - 81%. The highest number of alleles per genotypes/locus was detected using SSR primer set umc-1297, showing 9 alleles with an average of 3.11 alleles per genotype. Five SSR primers namely, umc-1173, umc-0455, umc-1257, umc-1289 and umc-1297 presented higher polymorphism rate by giving < 7 alleles. Cluster analysis grouped 36 maize genotypes according to ward’s method. The main clusters of 36 genotypes were found to split into 4 major clusters such as cluster (1, 2, 3 and 4). Cluster 4A exhibited high level of genetic diversity which indicated eight genotypes. Cluster 3A exhibited 2nd position in genetic diversity which also indicated eight genotypes.
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