PJB-2018-1056
Elicitor-Enhanced Phenolic Content and Antioxidant Activity in Date Palm Cell Suspension
Poornananda Madhava Naik
Abstract
The plants synthesize the phenolic and flavonoid compounds of having high antioxidant value. One of the in vitro culture approach, cell suspension culture elicitation offers a reliable method to produce nutraceutical important compounds possessing antioxidant properties. In this study, cell suspension cultures of date palm were grown in different concentrations of elicitors to determine the optimum cell growth conditions and accumulation of phenolic and flavonoid content. The date palm cell culture was experiment was carried by using Murashige & Skoog (MS) salts augmented with the 1.5 mg/l 2-isopentenyladenine (2-iP) and 10 mg/l 1-naphthaleneacetic acid (NAA) hormone combination, which are treated with the various elicitors such as pectin, yeast extract (YE), salicylic acid (SA), cadmium chloride (CdCl2) and silver nitrate (AgNO3) at 50, 100 and 200 mg/l concentrations. The effect of elicitors on cell suspension culture was determined in terms of packed cell volume (PCV), total phenolic, flavonoid content and antioxidant activity. The results revealed that enhanced PCV (12.33%), total phenolic content [317.986±28.743 mg gallic acid equivalents (GAE)/100 g of dry weight (DW)] and radical scavenging activity (86.09±4.54%) was obtained in the 50 mg/l SA treated cell suspension culture of MS medium. The maximum significant increase in total flavonoid content [157.286±20.775 mg quercetin equivalents (QE)/100 g of DW] was observed in the cell culture treated with 50 mg/l CdCl2. Therefore, these outcomes would be of great importance in the nutraceutical and agronomic industries.
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