PJB-2018-1066
LIGHT IRRADIATION AND METHYL JASMONATE APPLICATIONS IN VITIS VINIFERA CELLS: TOCOPHEROL ACCUMULATION
EMİNE SEMA ÇETİN
Abstract
In this study, the effects of light irradiation and methyl jasmonate (MJ) applications on the accumulation of tocopherols (α-, β-, γ- and δ-tocopherols) in grape cell suspension cultures was determined. Cell suspensions initiated from petiole tissue derived callus of Gamay, Kalecik Karası and Öküzgözü grape cultivars were used as plant materials. In the light irradiation treatment, cell suspensions were cultured in dark constantly (control) or exposed to visible light (10.000 lux) while MJ dissolved in ethanol was applied to cell suspension cultures at 10 µM concentration. Ethanol treatment was also included as a control. Cells were harvested at three days intervals until 15 day of cultures. Amounts of tocopherols (µg/g FCW), cell number (n) and cell viability (%) were determined in the harvested cells. In the present study, light irradiation and MJ treatments affected tocopherol accumulation in grape cells. Not only the dark culture condition but also the MJ treatment increased the tocopherol accumulations when compared to the light condition and non-exposed MJ application.
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