PJB-2018-1069
Antioxidant, anticancer activity and phytochemical analysis of green algae, Chaetomorpha collected from the Arabian Gulf
Samina hyder H Haq
Abstract
Background: Seaweeds are a group of marine multicellular algae; the presence of antioxidant phytochemical constituents in Seaweed Chaetomorpha extracts has received attention for their role in the prevention of human diseases. Hypothesis: This study explores the phytochemical constituents, antioxidant, and anticancer properties of the green algae, Chaetomorpha. Study Design: Energy dispersive x-ray spectroscopy (EDX), and Fourier-transform infrared spectroscopy (FT-IR) were performed to study the structure. Methods: Different concentrations of ethanol and aqueous extracts of Chaetomorpha were used to estimate antioxidant activity by DPPH radical scavenging activity and total flavonoid, phenolic, and tannins content assays. Anti-tumor activity against breast cancer cell lines (MCF-7 and MDA-MB-231) was assessed by MTT assay. Results: The EDX analysis indicated the presence of oxygen, silicon, and calcium as dominant elements. FTIR analysis further supported the presence of these compounds. Antioxidant assays indicated that the ethanol extracts of Chaetomorpha consisted of a total of 189.14 ± 0.99 mg QE/g flavonoid content, 21.92 ± 0.43 mg GAE/g phenolic content and 21.81 ± 0.04 mg GAE/g tannins content. The DPPH radical scavenging assay exhibited higher antioxidant activity IC50 (9.41 ± 0.54 mg/mL) in the ethanol extract. Moreover, it showed high anticancer activity by growth inhibition in MDA-MB-231 breast cancer cell line and low IC50 (225.18 ± 0.61 µg/mL). Conclusion: The results revealed that the type of Chaetomorpha studied here possesses very unique and novel constituents such as silicon, titanium and radium and it can act as a promising antioxidant and anticancer agent for future applications in food and medicine industries.
To Cite this article:
Download
