PJB-2018-1090
ANTIBACTERIAL POTENTIAL OF METHANOLIC AND WATER EXTRACTS OF QUERCUS INCANA AGAINST CLINICALLY IMPORTANT BACTERIA
Imtiaz Ali
Abstract
The misapplication of antibiotics has contributed to a dramatic increase of antibacterial resistance among clinically relevant bacterial species. New strategies beyond conventional antibiotics are required in the fight against infectious diseases. For this purpose, the antibacterial activity of the bark, fruit and leaves of Quercus incana, collected from Dir, Khyber Pakhtunkhwa Pakistan, against four human pathogenic bacteria, Escherichia coli, Klebsiella pneumoniae, Morganella morganii and Staphylococcus aureus was assessed. Crude extracts of the plant were prepared in two different solvents, i.e. methanol and water, evaporated under reduced pressure using Rotary vacuum evaporator. Serial dilutions were prepared from the crude extracts as; 500mg/ml, 250mg/ml, 125mg/ml, 62.5mg/ml, 15.75mg/ml and 3.5mg/ml. 0.05% (w/v) Ciprofloxacin was used as positive control and DMSO was used as negative control. All the extracted samples showed effective results against four bacterial isolates. The maximum zones of inhibition observed in methanolic leaves extracts for each bacterium were as follows: 22 mm Zone of Inhibition (ZOI) against S. aureus, 21mm ZOI against M. morganii, 19 mm ZOI against K. pneumoniae while 18mm ZOI against E.coli in bark extraction at the concentration of 500mg/ml. The E. coli was the most resistant strain of all throughout the research work. The results of the present study demonstrate that Quercus incana has substantial antibacterial potential.
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