PJB-2019-110
Enzymatic hydrolysis of Saccharum officinarum Lignocellulosic biomass by genetically modified hyperthermophilic cellulases
Ikram Ul Haq, Mariyum Riaz, Ali Nawaz Asad-Ur-Rehman, Hamid Mukhtar and Qurrat-Ul-Ain Syed
Abstract
The rapid exhaustion of fossil fuels and increasing environmental pollution due to burning of traditional fuels urge the scientists to look for alternative renewable energy resources. One of the important renewable energy sources is bioethanol. Bioethanol production from lignocellulosic substrate comprises of different steps. One of the important part of these steps is saccharification which yield fermentable sugars that can later be converted into ethanol by fermentation. In the present study, hyperthermophilic cellulolytic enzymes (Endoglucanase, Exoglucanase and β-Glucosidase) produced in genetically modified mesophilic host Escherichia coli BL-21 strain were used for hydrolysis of untreated and pretreated sugarcane bagasse samples. Alkaline peroxide treated bagasse was screened among differently pretreated sugarcane bagasse samples and saccharification yield of 26.64% was achieved by simultaneously adding 150 Units of Endo-1,4-β-glucanase, 300 Units of Exo-1,4-β-glucanase and 600 Units of β-1,4- Glucosidase for 3h at 80°C. Moreover, substrate concentration of 0.75% (w/v) yielded best hydrolysis rate of 35.19%. This optimization study resulted in 8.05 folds increase in the saccahrification yield which is an important step forward towards cheap production of fermentable saccharides.
To Cite this article:
Haq, I.U., M. Riaz, A. Nawaz, A.U. Rehman, H. Mukhtar and Q.U.A. Syed. 2019. Enzymatic hydrolysis of Saccharum officinarum Lignocellulosic biomass by genetically modified hyperthermophilic cellulases. Pak. J. Bot., 52(1): DOI: http://dx.doi.org/10.30848/PJB2020-1(26)
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