PJB-2019-114
MOLECULAR CLONING AND EXPRESSION ACCB GENE ENCODING BIOTIN CARBOXY-CARRIER PROTEIN OF CAMELLIA OLEIFERA (THEACEAE)
BAOMING WANG
Abstract
In this study, we cloned the CoaccB gene encoded biotin carboxy-carrier subunits of acetyl coenzyme A carboxylase (ACCase) of Camellia oleifera in. The cDNA was 1153 bp encoding 272 amino acids. Its molecular mass of the precursor and mature proteins was 28.93 and 22.02 kDa, respectively. Its amino acid sequence shared approximately 50% identity with those of accBs from some oil crops as well as E. coli. It contained Biotinyl_lipoyl_domains participiated in tranforming CO2 in ACCase enzemy, Biotin carboxyl carrier protein, biotinyl domain in lipid metabolism and carboxyltransferase inter site G227, M236KL, D245, Biotinylation motifs C231IIEAMKLMNEIE243, carboxylated by bicarbonate K237. Moreover, its transcript expression patterns were almost correlated with seed-oil contents, suggesting that it could potentially be as molecular markers for selection of higher oil production cultivars. Moreover, CoaccB displayed a trends of initially gradual increase, then slight decrease from stages of flower bud to seed mature. Together with those perfromances of CoaccC and CoaccD, we found these C. oleifera ACCase genes were coordinatedly expressed, and their vary trends were in accordance with changes of lipid and fat acid synthesis, and may be significant for seed-oil accumulation, thus be valuable for oil yield of C. oleifera cultivars.
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