PJB-2019-215
LeERF-1, an ethylene response factor gene from Lithospermum erythrorhizon, confers enhanced tolerance to cold and salt stresses in Arabidopsis
Hua Zhao, Rongjun Fang, Zhaoyue Wang, Aiqian Li, Shoucheng Huang, Jiangyan Fu, Zhongling Wen, Minkai Yang, Bao Liu, Guihua Lu, Runan Tian, Jinliang Qi and Yonghua Yang
Abstract
The ethylene responsive transcription factors (ERFs) play various functions in the processes of plant growth, development and myriad stress responses. We previously reported one light-regulated gene, LeERF-1, possibly paticipated in biosynthesis of secondary metabolites in medicinal plant Lithospermumerythrorhizon. Here, we further reported the function of LeERF-1 in cold and salt stress resistance by heterologous overexpressing in Arabidopsis. After cold treatment, the LeERF-1 overexpression lines (OE) exhibited better phenotype, higher survival rates, highersuperoxide dismutase (SOD) and peroxidase (POD) activities than that of the wild type (WT); while the malondialdehyde (MDA) concentrations of OE lines were significantly lower than that of WT. Exposing to 200 mM NaCl stress treatment, the germination and survival rates of OE lines were also remarkably higher than that of WT; The leaves of OE lines maintained relative lower proline content and had significantly lower Na+/ K+ ratio compared to that of WT. These findings collectively indicate that over-expression of LeERF-1 confers improved cold and salt resistance in transgenic Arabidopsis
To Cite this article:
Zhao, H., R. Fang, Z. Wang, A. Li, S. Huang, J. Fu, Z. Wen, M. Yang, B. Liu, G. Lu, R. Tian, J. Qi and Y. Yang. 2021. LeERF-1, an ethylene response factor gene from Lithospermum erythrorhizon, confers enhanced tolerance to cold and salt stresses in Arabidopsis. Pak. J. Bot., 53(1): DOI: http://dx.doi.org/10.30848/PJB2021-1(4)
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