PJB-2019-285
Comparative microRNA profiling reveals the cold response mechanisms in contrasting tobacco cultivars
Risheng Hu
Abstract
Cold in late spring seriously affects the yield and quality of tobacco leaves. It’s widely known that microRNAs (miRNAs) are involved in the plant growth, development and response to various stresses. However, the role of miRNAs in tobacco response to cold stress remains elucidated. In the present study, comparative analysis of miRNA profile in cold-tolerant tobacco cultivar NC567 and cold-sensitive Taiyan8 with and without cold treatment was performed. More than 22 M raw reads were obtained for each sample. The alignment against the miRbase identified 164 conserved miRNAs. A total of 25 cold-regulated (COR) conserved miRNAs in NC567 and 13 in Taiyan8 were discovered. Further analysis revealed the down-regulation of 15 nta-miR169 family members only in NC567, up-regulation of 7 nta-miR399 members just in Taiyan8, and the contrasting expression patterns of miR6154b and a in two cultivars. Combined with Gene Ontology (GO) analysis of target genes, the cold response model was postulated, and demonstrated the positive role of miR169 family members and the negative effect of miR399 as well as the conserved mechanism of miR477a, miR169t and miR156f in tobacco response to cold stress. The study provides important information for understanding and improving the tobacco tolerance to cold stress.
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