PJB-2019-323
GENETICS VARIATION MELON (Cucumis meloL.) TACAPA CULTIVAR USING RANDOM AMPLIFIED POLYMORPHIC DNA (RAPD)
BUDI SETIADI DARYONO
Abstract
Melon (Cucumis meloL.) cultivar TACAPA is a new melon cultivar that has potential to be an important local melon seed in Indonesia. The phenotype of TACAPA cultivar has been indentified, but its genetic variation has not been studied yet. This study was aimed to analyze the genetic variation of melon cultivar TACAPA and compared with PI 371795 and Action 434 as ancestor of TACAPA, and 9 cultivars from the same ancestor, 3 commercial melons and 1 cultivar of cucumber. PCR-RAPD method with 5 random primers was used to evaluate genetic variation in melon. Level of genetic similarity was determined by formula simple matching coefficient (SSM) and the dendogram constructed by clustering analysis with unweighted pair-group using arithmatic averages (UPGMA). Result of PCR-RAPD with 5 primers (PMAR, OPAK-16, OPAX-16, OPA-07 and OPD-13) produced 59 loci and it contained 51 polymorphic DNA bands and 8 monomorphic DNA bands. Results of genetic variation analysis suggested that TACAPA and 14 melon cultivars differ from cucumber. Cultivar TACAPA had genetic markers that can be distinguised from other melon cultivars, however TACAPA has a high similarity (97,143 %) with SONYA, one of commercial melons.
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