PJB-2019-369
Enhanced Accumulation of Silymarin in elicited suspension cultures of S. marianum
zara jabeen
Abstract
Liver diseases are the most common menace now a days in Pakistan. Herbal medication gains attention in this respect and among all medicinal plants, Silybum marianum (milk thistle) considered most well-studied plant against liver disorders. Silymarin is a biomarker of Silybum marianum extracted from seeds and used against liver diseases. But there are constraints regarding production of silymarin production from seeds of milk thistle. In this context, present study was planned to hyper produce the therapeutically important secondary metabolite (silymarin) by using biotechnological approaches to fulfil the scarce demand of silymarin. Silymarin is extracted from Silybum marianum which is a most effective and commonly used medicinal plant for the treatment of liver disorders. Owing to the less production through traditional cultivation techniques and enhanced demand of silymarin for medicinal purposes, there is dire need to hyper-produce silymarin through tissue culture techniques. For this purpose, suspension cultures were established from pre-optimized callus in agar free medium. Methyl Jasmonate and silver nitrate (elicitors) were added at different molar concentrations (10, 50, and 100mM) and samples were collected for three consecutive days. Culture extracts were harvested and characterized for their silymarin content through spectrophotometric and HPLC analyses. After the completion of experiment, spectrophotometric as well as HPLC analysis showed that elicited suspension cultures extracts have great potential to enhance the silymarin production as compared to un-treated cultures. Among elicitors methyl jasmonate showed significant potential at 10mM concentration for the maximum production of silymarin after 24 hours of the treatment.
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