PJB-2019-372
IMPACT OF PHOSPHORUS LEVELS AND ISOLATED MYCORRHIZA FROM CALCAREOUS SOIL ON BIOCHEMICAL ACTIVITY OF ZEA MAYS
AMEL TAMMAM
Abstract
This study aimed to explore the effect of mycorrhizal inoculation along with different phosphorus concentrations on growth, activities of alkaline phosphatases (ALP) in producing and supplying inorganic phosphate for the various biochemical processes that require it during the growth of roots and shoots of maize plant and proton-pumping ATPase (H+-ATPase) of the maize plant plasma membrane generates the proton motive force across the plasma membrane that is necessary to activate most of the ion and metabolite transport in root and shoot tissues of maize plants on calcareous soil. A greenhouse study to investigate the effect of mycorrhizal inoculation with five phosphorus levels 0, 15, 30, 60 and 120 mg P kg-1soil (in the form of KH2PO4) on growth parameters and biochemical reactions. The mycorrhizal inoculation and phosphate significantly increased plant growth, P concentrations, total soluble protein, and proline contents. Increase in phosphatase activities and growth responses of the Zea mays plants to AMF symbiosis were directly proportional to the levels of the mycorrhizal colonization. The proton-pumping ATPase (H+-ATPase) of the plant plasma membrane generates the proton motive force across the plasma membrane that is necessary to activate most of the ion and metabolite transport in root and shoot tissues compared with non- mycorrhizal maize plants. This stimulation was related to the level of the mycorrhizal colonization in the root tissues. The results confirmed that mycorrhizal colonization could improve growth and nutrition of the maize plant in calcareous soil of Egypt. Four native species of mycorrhizal fungi were isolated from the rhizosphere of Zea mays grown on calcareous soils (pH 8.4). Based on size, color and cell wall structure of spores, these species were identified as Glomus, Acaulospora, Scutellospora and Entrophospora Positive PCR results were for family Glomaceae and Acaulosporaceae were obtained.
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