PJB-2019-385
Genome-wide computational analysis of WRKY transcription factor gene family from Sorghum bicolor
Erum Yasmeen
Abstract
The cellular activities, stress responses and developmental physiology of a plant are regulated by regulatory proteins called transcription factors (TFs). WRKY is a gene family in which these proteins play a vital role in up and down regulating various genes to modulate the expression in response to several stimuli. The DNA-binding domain of these proteins is a conserved heptapeptide sequence WRKYGQK. We retrieved the protein sequence of 97 candidates of WRKY gene family of Sorghum bicolor. These 97 proteins are grouped in three subfamilies according to their structural properties and number of domains. These WRKY TFs are located on 10 chromosomes of Sorghum bicolor with uneven distribution. We characterized diversity in amino acid residues in WRKY domain and found conserved motifs from WRKY proteins. Moreover, gene structure analysis showed the distribution of introns and exons on each gene which explains the functional stability in context of dark matter and coding region correlation. The promoter analysis indicated the distribution of 6 cis-regulatory elements were located on 1 kb of the promoter sequence. Synteny analysis showed strong structural and functional relationship among the WRKY proteins of Arabidopsis and Sorghum. This study will be helpful in providing the in silico genomic and proteomic information about the WRKY transcriptional factor in Sorghum bicolor and Arabidopsis thaliana. Moreover, the following findings will help in understanding WRKY family for their diverse role in plant stress and development in sorghum.
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