PJB-2019-396
FUNGAL FLORA OF HORDEUM VULGARE L. AND EFFECT OF ULTRA VIOLET C RADIATIONS ON IT
shahnaz Dawar
Abstract
Twenty four fungal species with 13 genera were identified from 12 samples of barley collected from different localities of Pakistan. These fungi were detected by three methods namely, blotter, agar plate and deep freezing methods described by International Seed Testing Association (ISTA). Fungi identified as Absidia sp., Alternaria alternata, A.brassiciciola, *A. solani, *A. raphanii, Aspergillus clavatus, A.flavus, A.fumigatus, A,terreus, A.wentii, A.niger, *A.sclerotium, *Botrytis sp., Chaetomium sp., Cladosporium sp., Drechslera hawaiiensis, Fusarium solani, Mucor hiemalis, Penicillium digitatum, Phoma eupyrena, Rhizopus stolonifer, Trichoderma hamatum and T. harzianum. The incidence of fungal flora was comparatively higher in agar plate method as compared to blotter and deep freezing methods. Surface sterilized of seed reduced the incidence of saprophytic fungi while, the incidence of saprophytic fungi was higher in non- sterilized condition. Aspergillus species was observed in all samples tested particularly A. niger. The most frequently isolated and identified fungi were Alternaria alternata, Aspergillus flavus, A.fumigatus, A.niger, A.terreus, Rhizopus stolonifer, Mucor sp. and Trichoderma harzianum. Barley seeds sample collected from Kashmir showed maximum number of fungi were further subjected to treat with ultra violet C (UVC) for different time period. Results showed that incidence of fungi greatly reduces after 30 and 60 days of storage. However, Alternaria alternata and Aspergillus fumigatus have no effect after 60 days of storage at room temperature
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