PJB-2019-441
COMPARISON OF HEALTHY AND HLB-AFFECTED CITRUS CULTIVARS THROUGH GLOBAL MASS SPECTROSCOPIC ANALYSIS (LC-MS)
KHADIJA GILANI
Abstract
Haunglongbing (HLB) or Citrus Greening is one of the major bacterial diseases responsible of declining production rate of citrus in Pakistan, as well as world widely. The main Protobectia (ɑ subdivision class), Candidatus Liberibecter is the main source of HLB disease. Gram negative, fastidious nature, uncultured bacteria grows in the phloem sieve tubes elements of plant cells and generally transmitted through Psyllid, Diaphorina citri, which infested the bacterium mainly through grafting of citrus trees. This study was used to determine the profile of vast range of targeted and untargeted metabolites through LC-MS. Results indicated that there was a total of 13,752 features were detected combined through positive and negative ion mode. Sixty five percent of the features (metabolites instead of features) were detected in the negative ion mode and 35% from the positive ion mode. Addition of known metabolites with unknown negative metabolites in leaves, the PLS-DA separations (known and unknown features) are highly significant for both negative and positive ions when leaf and pulp samples are combined. The result of the permutation test was p
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