PJB-2019-463
SCREENING ALLELOCHEMICAL COMPOUNDS SECRETED BY LOCAL ISOLATES OF PHOSPHATE SOLUBILIZING FUNGI (PSF) BY GC-MS ANALYSIS
AZHER HAMEED FARAJ AL-TAIE
Abstract
Local fungal isolates (LF) of Phosphate Solubilizing Fungi (PSF) was isolated from Rhizosphere soil of plants grown in Wasit province (south-central of Iraq) and some organic waste. This study aimed to screen the liquid fermentation content of LF of (PSF). The results showed that Aspergillus niger was most appearance and frequency fungi in all samples. Isolates of A. niger isolates AD-A2 and AG-B1 formed a halo-clear zone around their colonies as results of its phosphate-solubilizing ability in PVK and NBRIP solid artificial media after 5 days of incubation at 27°C. The study indicated that the pH of the final product from the fermentation of isolate AD-11 3.52 and 4.71 followed by the isolate AD-A2 with 5.05 and 5.30 in both PVK and NBRIP broth medium as compared to the control where it remained constant around pH 5.75 and 6.61 respectively. Metabolic releases from PSF isolates caused increase in root length and shoot height of muskmelon by14 ml of fungal filtrate for 10 days of incubation at 27 °C. A. niger AD-11 was able to increase in shoot length and root height most than other PSF isolates 14.5 and 9.5 mm compared with free of fungal inoculants 6.8 and 4.3mm respectively. While Trichoderma hamatum T-113 have a similar effect to the A. niger isolates with 10.8 and 8.3 mm in shoot length and root height respectively. The decreasing of pH of submerged fermentation that incubated at 27°C for 14 days on a shaker incubator 120 rpm to be the main factor governing organic compounds producing which carried out to identify by GC-MS analysis. Many compounds are formed by submerged fermentation of Aspergillus isolates were identified in as following: 1,3-Dioxan-5-ol (96.47%); Octanoic acid, 4-chlorobenzyl ester (0.14%); 1,3,5-Cycloheptatriene (0.58%); Crotonic acid (2.64%); 2-Propanone, 1,1-diethoxy (0.18%); 2-Butanone, 4-hydroxy (13.11%); 1-Tetrazol-2-ylethanone (86.4%); Propanethioamide (0.49%); Acetic acid, ethyl ester; Acetidin (100%); Methylolacetone (100%) and Trichoderma hamatum (T-113) were identified: Ethane, 1,1-diethoxy (100%) and 2-Butanone, 4-hydroxy-(100%).
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