PJB-2019-498
Induction of Tetraploidy in Phalaenopsis Luchia Pink by Colchicine In Vitro
Apriliana Dyah Prawestri
Abstract
Polyploidy is a condition of possessing more than two complete sets of chromosomes and is common among plants. Polyploidization plays an important role in plant breeding and improvements of crops and ornamental plants, such as orchids. Polyploid induction can be done using antimitotic agents, such as colchicine. The effect of colchicine on the protocorm of Phalaenopsis Luchia Pink cultured in vitro was investigated. Protocorms in ½ MS liquid media were subjected to colchicine treatment of 0.00, 0.025, 0.05, 0.1 and 0.2% (w/v) concentrations for 6, 12, 24, 48 and 72 hours. All colchicine treated-protocorms were cultured on ½ MS medium without growth regulator for 8 weeks, then subcultured on ½ MS + 2 ppm BAP + 0.5 ppm NAA for 16 weeks. The result showed that although colchicine increased the formation of new protocorm but it decreased the protocorm survival and plantlet regeneration. The most effective treatment to regenerate tetraploids in Phalaenopsis Luchia Pink Colchicine was 0.2% of colchicine for 12 hours. Colchicine also increased the size of stomata and epidermal cell of plantlets but decreased stomatal density.
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