PJB-2019-506
DELETERIOUS EFFECTS OF ARSENIC CONTAMINATION ON PENICILLIUM SURVIVAL. RASHIDA SULTANA 1, MALEEHA UMBER * 2, SHANZAH KIRAN 3,AYESHA KHALID 3 AND MIRZA NASEER AHMAD3 1. Lecturer, Department of Botany, Faculty of Life Sciences, Nusrat Jahan College Rab
Rashida Sultana
Abstract
Current study was done at Botany department, Nusrat Jahan College Rabwah Pakistan to examine detrimental effects of arsenic stress on penicillium extracted from decayed orange. Sodium arsenite was prepared in following concentrations1mg/L, 2mg/L, 3mg/L, 4mg/L, 5mg/L,6mg/L, 7mg/L, 8mg/L, 9mg/L and 10mg/L and small amount of each concentration was then applied to penicillium which was taken from decayed orange and maintained on CLED media to determine zone of inhibition through disk, well and spread method. Stress applied and control (without arsenic stress) petri plates were incubated for 24 hours at 37◦c. After competition of incubation, zones of inhibition were observed and measured. Results of our research have shown that penicillium growth has been adversely inhibited with increase in arsenic stress. Among all three above mentioned methods, spread method has most drastic growth retarding effects on penicillium. Key Words: penicillium, arsenic, CLED, well method, spread method, disk method
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