PJB-2019-518
Impact of drought stress on the physiology and stress- responsive genes regulation of diverse soybean cultivars.
Tian Yang
Abstract
Soybean (Glycine max L. Merr.) is a major economic oilseed crop. Its growth and productivity are adversely affected by water deficit. Identification of germplasm with high drought-tolerant potential is essential for soybean breeding to alleviate water shortage. In the present study, we first evaluated drought tolerance of eight soybean cultivars based on physiological parameters including relative water content, osmoprotectants accumulation, antioxidant activity, relative conductivity and malondialdehyde content under both normal and drought conditions. The data indicated that drought tolerance widely varied among all the examined cultivars. QB-21 was the strongest drought-tolerant cultivar with highest osmotic adjustment ability and antioxidant activity, whereas QB-67 was the most sensitive phenotype we observed. Next, transcriptional changes of eight selected stress-responsive genes were analyzed in the two contrasting phenotypes QB-21 and QB-67 using real-time quantitative PCR. We found that upon drought condition, the expression induction of stress-related genes in QB-21 was quicker and more intense, while in QB-67 the response was delayed and limited. The qPCR results confirmed that up-regulated expression of these candidate genes resulted in elevated drought tolerance in soybean. Taken together, the information we obtained form this research provide valuable resource for breeding drought-tolerant soybean cultivar.
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