PJB-2019-540
Anti-Cancer and Antimicrobial Efficacy of Selected Medicinal Plants of Central Karakorum National Park (CKNP) Region Gilgit-Baltistan, Pakistan
Qamar Abbas
Abstract
In the current investigation five mountain herbs were assessed for anticancer and antimicrobial potential. Two type of extracts including dicholoromethane (DCM) and methanolic extract were screened for cytotoxic activities against cancer cell lines (HeLa). Result showed that methanolic extract exhibited high anti-cancer properties as compared to DCM extract. Highly significant anticancer potential (IC50 ± SD µg/ml >100) of methanolic extracts were found in both Salvia nubicola and Delphinium brunonianum respectively. Saussurea simpsoniana DCM (4.80±0.401) and S. nubicola DCM (18.46± 0.53) extracts have cytotoxic potentials while other plant species including Pulsatilla wallichiana DCM (12.002±0.22) and Gentiana tianschanica DCM (3.33±0.30) showed moderate cytotoxic effect towards Heler Cells lines. Results pertaining to antimicrobial activity showed that herbs extracts possess some potential against bacteria and fungi. When tested by the disc-diffusion assay, the methanolic extracts of the plants Gentianodis tianschanica has no antibacterial potential against tested bacterial strains, while S. nubicola showed minimum inhibition potential against Shigella flexenari (9.63%), P. wallichiana against Pseudomonos areuginosa (4.39%), S. simpsoniana against Staphylococcus aureus (16.99%) and Salmonella typhhi (30.21%), and D. brunonianum against Shigella flexenara (12.80%) and Escherichia coli (3.89%). Bacterial strain E. coli and Bacillus subtilis showed resistance against all tested plant extract. For assessment of antifungal activity result revealed that Candida albican resistant against tested extract. However, G. tianschanica showed activity against Fusarium lini (30%) while S. nubicola depicted significant activity against Trichphyton rubrum (80%) and Microsporum canis (60%). The results of P. wallichiana water extract also showed significant activity against Trichphyton rubrum (35%) and Microsporum canis (70%), while with methanolic extract P. wallichiana also showed significant activity against T. rubrum (60%). S. simpsoniana methanolic extract showed significant activity against T. rubrum (60%) and Fusarium lini (30%). D. brunonianum methanolic extract showed significant activity against T. rubrum (60%).
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