PJB-2019-542
EVALUATING THE EFFECT OF COMBINED PLANT EXTRACTS ON α-AMYLASE AND α-GLUCOSIDASE INHIBITION ACTIVITY AS ANTIDIABETIC AGENTS
OSAMA ABDULMUNEM K
Abstract
This study aims to screen the in vitro antidiabetic activity of seven plant extracts. The bioactivity of the extracts was correlated through a GC-MS-based analysis, while extracts with 50% inhibition activity were reported. The α-amylase inhibitory activities of the chloroform extracts of Averrhoa bilimbi, Andrographis paniculata, Eurycoma longifolia, and Punica granatum exhibited more than 50% inhibition (50.55, 68.24, 82.42 and 69.73% respectively), while chloroform extracts of A. paniculata, Orthosiphon stamineus and P. granatum exhibited 90.48, 52.00 and 62.00% of α-glucosidase inhibitory effects, respectively. Only ethanolic and methanolic extracts of E. longifolia exhibited α-glucosidase inhibitory effects of 57.80 and 53.31%, respectively. A combination of the ethanolic fraction of E. longifolia with the chloroform fraction of P. granatum at a ratio of 2:1 exhibited the highest α-glucosidase inhibition rate of 148.06%, while a combination of the ethanolic fraction of E. longifolia with the chloroform fraction of O. stamineus at the same ratio exhibited α-glucosidase inhibition rate of 137.43%. A phytochemical analysis revealed the presence of fatty acids, with palmitic acid recording the highest percentage (100%) in the O. stamineus extract. The observed α-amylase and α-glucosidase inhibitory activities of these plant extracts suggest their potential usage as alternative sources of antidiabetic agents.
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