PJB-2019-558
GENOMIC ANALYSIS OF REGIONS FOR MORPHOLOGICAL, PHYSIOLOGICAL AND BIOCHEMICAL ATTRIBUTES UNDER SALT STRESS IN RECOMBINANT INBRED LINES OF WHEAT (Triticum aestivum L.)
NAZIMA BATOOL
Abstract
Salt stress is a major limitation to plant growth, nutritional imbalance, ion toxicity and crop production around the globe. There are salt tolerant landraces and traditional varities of wheat but with limited avaliable information related to their molecular mechanisms. In order to reveal the genetic basis of morphological, physiological and biochemical attributes of wheat under salt stress condition, recombinant inbred lines (RILs) were derived by crossing two bread wheat cultivars, namely Pasban90 and Frontana. A Linkage map was constructed by utilizing 202 polymorphic simple sequence repeats (SSR) markers spanned on a total of 2630.1 cM. B genome contributed to the highest number of QTLs under salt stress condition. Xgwm70and Xbarc361 mapped on chromosome 6B was linked with Total chlorophyll, water potential and sodium content. Linked QTLs for membrane stability index, water potential, and root potassium on chromosome 3D were segregating with Xgwm71 and Xgwm341. QTL for water potential under hydroponics control condition (QWP.5B-CH) with LOD value of 3.4 and R2 of 17 were identified on chromosome 5B with an increasing allele from Pasban 90. The increasing allele for all these QTL was originated from parent Pasban90. These identified QTLs may serve as potential targets for high QTL mapping and marker assisted selection. Novel QTLs for water potential, osmotic potential, relative water content and chlorophyll content were also identified.
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