PJB-2019-567
Cloning, Sequencing and In- silico analysis of Germin-like protein Gene 12-3 promoter from indica Rice c.v Swat-1.
Rashid Ahmad
Abstract
ABSTRACT Germin and germin-like proteins (GLPs) are a class of plant proteins that belong to the superfamily of plant’s proteins called "Cupins". They are reported to express in different plants organs like nectar glands, roots, leaves and seeds. Gene expression of germins and germin-like proteins is also been reported to be included in plant defense against pathogenic attack and in response to different environmental stress conditions. In the present study, promoter analysis of germin-like protein 12-3 (OsGLP12-3) was carried out in the rice cultivar Swat-1 as it explains functioning, regulation and response of the particular gene. The OsGLP12-3 promoter region, 1863bp, was amplified using genomic DNA of Oryza sativa L. cultivar Swat-1. The amplified products were purified, quantified and cloned using Topo cloning technology and then subject to sequencing. In silico comparative analysis of the sequences of OsGLP12-3 promoters from Nipponbare and Swat 1 were done for the identification of cis-acting regulatory elements by using the online tool PlantCARE database. About 24 cis-acting regulatory elements were identified in the OsGLP12-3 promoter region having a diverse range of functions. Each element was found to exist with a distant copy number at a distant specific location in two varieties of rice i-e. Nipphonbare which is the standard and Swat-1. In the study, OsGLP12-3 promoter region was found to contain enormous upstream regulatory elements confined to give defense against biotic and abiotic stresses. Upstream regulatory region (promoter) of OsGLP12-3 was assessed using different bioinformatics tools to find out the regulatory aspect of the particular element c.v Swat-1
To Cite this article:
Download
