PJB-2019-572
SMOKE-ISOLATED KARRIKINOLIDE ENHANCES α-AMYLASE ACTIVITY OF SEEDS OF PANAX QUINQUEFOLIUS BY ENHANCING ENDOGENOUS NITRIC OXIDE AND HYDROGEN PEROXIDE LEVELS
JIE ZHOU
Abstract
Smoke-water (SW) and biologically-active compound karrikinolide (KAR1) have shown stimulating effects on numerous species of seed germination. However little information have been reported on signal transduction mechanism of KAR1-induced seed germination. This study was focused on investigating the roles of NO and H2O2 play during KAR1-induced seed germination of Panax quinquefolius. Relevant scavengers and inhibitors have been used to investigate the signal transduction mechanism involved in KAR1-induced seed germination. KAR1 induced H2O2 generation, triggered NO accumulation and enhanced α-amylase activity and seed germination in P. quinquefolius. KAR1-induced increasing of α-amylase activity was blocked by inhibitors of NO and H2O2, showing that NO and H2O2 play essential roles in KAR1-induced increasing of α-amylase activity. KAR1-induced generation of NO and H2O2 was inhibited by H2O2 inhibitors, suggesting H2O2 and NO acted at least partially in the same signaling pathway, within which H2O2 was localized in the upstream of NO. H2O2-induced increase of α-amylase activity was blocked by NO inhibitors, implying H2O2 mediated the KAR1-induced activity of α-amylase through a NO-dependent signaling pathway in P. quinquefolius seeds. H2O2 mediates KAR1-induced increase of α-amylase activity by NO-dependent pathway, which help us preliminarily understand the mechanisms involved in KAR1-induced seed germination and provide useful information for the production of P. quinquefolius.
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