PJB-2019-620
Exogenous nitric oxide enhanced proanthocyanidins accumulation in fiber during ovule culture in vitro for brown cotton
Xu Sun
Abstract
Abstract: Nitric oxide (NO) is a vitally important secondary biological messenger during the plant secondary metabolism. Proanthocyanidins, a class of flavonoids, are important secondary compounds found common in cotton. But the underlying mechanism of PAs synthesis in cotton still largely unclear. To investigate whether exogenous NO role during the biosynthesis of PAs in cotton, we in vitro cultured cotton ovules fibers from a brown fiber line and treated with sodium nitroprusside (SNP, NO donor). We determined the contents of PAs and the expression of its key enzyme genes of GhCHS, GhF3H, GhDFR, GhANS, GhANR, and GhTT12. Our results showed that 100 μmol·L-1 SNP treatment significantly increased the concentrations of PAs and the total contents of flavonoids in brown fiber. The PAs concentrations reached the highest level at the stage of 15 DAC (days after culture). Furthermore, the expression levels of key enzyme genes GhCHS, GhF3H, GhDFR, GhANS, GhANR, and GhTT12 were enhanced with the SNP treatment. Our results provide the comprehensions into the role of exogenous NO in the biosynthesis of brown pigment in fiber.
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