PJB-2019-639
Effect of lignin supplementation in corn stalk cultivation substrate on Lentinula edodes growth
Fei Wang
Abstract
Lentinula edodes is an economically important edible fungi usually cultured on wood logs and sawdust substrate, from which it obtains the nutrients needed for growth by degrading lignin, cellulose and hemicellulose. In this study, the transparent hydrolysis ring method and growth rate in corn stalk medium was used to screen for L. edodes strains grown on corn stalk substrate with high efficiency. Five formulas were designed with different proportions of corn stalk as the primary cultivation substrate. To investigate the effect of supplementing the corn stalk substrate with lignin on L. edodes growth, specific proportions of lignin were supplemented into each formula such that the lignin content in all the formulas was identical to that of the control (CK) substrate (32.77%), which was mainly composed of sawdust. The results showed that strain No. 1 exhibited a high utilization of corn stalk, and as the amount of corn stalk in the cultivation substrate increased, the hemicellulose and cellulose contents decreased. In addition, the mushroom yield and biological conversion efficiency first increased and then decreased, with Formula 1 and 2 performing better than the CK in terms of growth cycle, yield, and biological efficiency (BE). Formula 2, which consisted of sawdust (36%), corn stalk (36%), and lignin (8%), with the carbon to nitrogen ratio (C/N) of 15.71, showed the highest mushroom yield at 194.28±0.25 g/bag, which was higher than CK by 28.14%. Furthermore, Formula 2 resulted in a BE of 64.76±1.19%, which was higher than that of the CK by 14.22%, as well as a cultivation cycle of 102.12±0.69 days, which was 2.88 days shorter than that of the CK. Moreover, the lignin was not the only factor that influenced mushroom growth, the lower C/N, cellulose and hemicellulose contents of formula significantly decreasing yield and BE and prolonging a cultivation cycle. These results demonstrate the usefulness of the rational utilization of crop straw in edible fungi cultivation.
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