PJB-2019-645
DETECTION, IN SILICO ANALYSIS AND MOLECULAR DIVERSITY OF PHYTOPLAMAS FROM SOLANACEOUS CROPS IN TURKEY
mustafa usta
Abstract
Phytoplasma-like symptoms of leaf yellowing and calyx malformation were observed in eggplant (Solanum melongena L.), upward leaves and fruit malformation in pepper (Capsicum annuum L.) and aerial tuber formation in potato (S. tuberosum L.) during the survey performed in the late season (August to September) of 2015 and 2016 in Van province (Turkey). A total of 100 samples were tested by nested-PCR using universal primer pairs to assess the sanitary status of the Solanecaeus crops and to characterize the Phytoplasma isolates. Among them, 7 samples resulted a 1.25 kb DNA fragment and 5 (2 eggplants, 2 peppers and 1 potato) were molecularly characterised (Accession no: KY579357, KT595210, MF564267, MF564266 and MH683601). BLAST and computer-simulated restriction fragment length polymorphism (RFLP) analysis of 16SrRNA genes were revealed the presence of two distinct Phytoplasma infections in Solanecaeus crops:‘Candidatus Phytoplasma trifolii’ (Ca. P. trifolii) a member of the clover proliferation group (16SrVI group) and subgroup A, and ‘Candidatus Phytoplasma solani’ (Ca. P. solani) a member of the Stolbur group (16SrXII) and subgroup A. The virtual RFLP analysis and calculated coefficients of RFLP pattern similarities were further revealed a remarkable genetic diversity among the ‘Ca. P. solani’ isolates infecting pepper (similarity coefficient of 0.90) and eggplant (similarity coefficients of 0.98 and 1.00) at the same geographical area. This is the first report of naturally occurence of ‘Ca. P. trifolii’ in potato from Eastern Anatolia Region/Turkey.
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