PJB-2019-653
PRODUCTION OF MEDICINALLY IMPORTANT LOW MOLECULAR WEIGHT ANTIOXIDANT SECONDARY METABOLITES IN CALLUS CULTURES OF PRUNELLA VULGARIS L.
HINA FAZAL
Abstract
Recently, the interest in natural antioxidant secondary metabolites has increased tremendously to cure various diseases. Prunella vulgaris L. is an emerging medicinal herb containing various polyphenolics which is used for wound healing and antitumor properties. In this study, plant growth regulators-induced callus cultures were exploited for growth kinetics, biomass accumulation and production of secondary metabolites. Most of the applied PGRs induced calli from leaf explants on Murashige and Skoog (MS) media, but the callus characteristics were found different. A growth curve was established for each applied PGR for a period of 49 days which showed a distinct lag (7-27 days), log (28-42 days) and decline phases (43-49 days). Among different PGRs, the combination of naphthalene acetic acid (NAA) and 6-benzyleadenine (BA; 0.5 and 2.0 mg l-1) produced maximum fresh (6.32 FW-g/100 ml) and dry biomass (0.75 DW-g/100 ml) as compared to control. The maximum production of superoxide dismutase (0.0154 FW-nM/min/mg)was observed on media containing combination of NAA and BA (1.5 mg l-1), while peroxidase enzyme (0.366 FW-nM/min/mg) was higher at0.5 mg l-1 NAA and 2, 4-dichlorophenoxy acetic acid. The combination of NAA and BA (1.5 and 2.0 mg l-1) enhanced the production of phenolics (18.83 GAE-mg/g-DW) and flavonoids content (18.05 RE-mg/g-DW) than control. Moreover, 1.0 and 2.0 mg l-1 of NAA were found supportive for maximum antioxidant activity (87.4%) and content of total protein (716 µg BSAE/mg-DW). This study will help in the establishment of cell culture in bioreactor and production of antioxidant secondary metabolites for commercial uses.
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