PJB-2019-655
HIGH MOLECULAR WEIGHT GLUTENIN COMPOSITION OF WHEAT LANDRACES REPORTED FROM NORTHREN HILLY AREAS OF PAKISTAN
Syed Fazal Wadood
Abstract
To evaluate inconsistency in grains of wheat, 62 landraces and 10 cultivars of wheat collected from northern hilly areas of Pakistan were analyzed for bread making quality. This was based on HMW-Glutenin composition through SDS-PAGE. A total of 7 HMW-GS alleles were found at Glu-A1, Glu-B1 and Glu-D1 loci resulting in 4 HMW-GS unique combinations. The HMW-GS combination “Null, 6+8, 2+12” was the dominant phenotype, which was identified in 62.50% of Pakistani wheat genotypes. Similarly the dominant alleles at Glu-A1, Glu-B1 and Glu-D1 loci were Null (62.50%), 6+8 (86.11%) and 2+12 (86.11%), respectively. Quality score of genotypes were ranked from 4 to 9. The high quality score of 9 was found in 13.86% genotypes (Cultivars = 1, 7+9, 5+10). Dendrogram based on genetic similarity matrix of HMW-GS delineated genotypes into two Clusters; A and B with three sub-clusters for the cluster A. Genotypes were less diverse, form groups with a 100% similarity showing strong linkage. This confirmed utilization of the shared gene pool, imitating loss of genetic diversity. In order to stop such genetic erosion, it is obligatory to screen out quality wheat lines, used in the present breeding activities to preserve them for future quality bread wheat cultivation strategies.
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